RNA Oligonucleotides

General Characteristics.

Quality

- fully deprotected and desalted, purified by PAGE
or RP-HPLC on request.
- it is highly recommended to use purified RNA for
oligos > 30 mers.

 

Form

- RNA and 2' O-Methyl mixed oligos are sent dry
- Quantity (OD and nmoles) and concentration (µM and µg/µl)

Guarantee

- Guaranteed sequence, quality and quantity
Please see yield table above for guaranteed minimum yields

Documentation

All Oligos are delivered with an Oligonucleotide Technical Data Sheet that can also be sent through e-mail

Storage

Although oligonucleotides are stable at 4°C for up to 2 weeks, store at -20°C. Repetitive freeze-thaw cycles should be avoided by storing as aliquots. For long-term storage, RNA oligos should be dried. 

Quality

 
Desalted
 Synthesis Scale  0.05 µmole  0.2 µmole  1.0 µmole
 Standard RNA  X  X  X
 Phosphorothioate linkages (s-oligos)  X  X X

Guaranteed Yields (7-45 mers):

 

Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole
Desalted 5 OD (150µg) 12 OD (360µg) 40 OD (1200µg)
RP-HPLC 1 OD (30µg) 2.5 OD (75µg) 13 OD (390µg)
PAGE 0.5 OD (15µg) 1 OD (30µg) 5 OD (150µg)

Purification:

Quality

Desalted
Synthesis Scale 0.05 µmole 0.2 µmole 1.0 µmole
RP-HPLC X X X
PAGE X X X

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